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anti human cd45 alexa fluor 647  (Bio-Rad)


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    Structured Review

    Bio-Rad anti human cd45 alexa fluor 647
    Anti Human Cd45 Alexa Fluor 647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd45+alexa+fluor+647/Mouse+anti+Human+CD45/pm41350390-209-51-58
    Average 93 stars, based on 142 article reviews
    anti human cd45 alexa fluor 647 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance
    Article Snippet: .. Patient slides in were fixed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [ ]. .. EpCAM (Thermo, 14-9326-82) was included in the pan-cytokeratin cocktail mixture to make an “EPI-cocktail”.

    Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance.
    Article Snippet: .. Patient slides in Fig. 1 were fixed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4’,6- diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [46]. .. EpCAM (Thermo, 14-9326-82) was included in the pan-cytokeratin cocktail mixture to make an “EPI-cocktail”.

    Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance
    Article Snippet: .. Immuno uorescent staining: Patient slides in Figure 1 were xed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4',6-diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [28]. .. EpCAM (Thermo, 14-9326-82) was included in the pan-cytokeratin cocktail mixture to make an “EPIcocktail”.

    Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance
    Article Snippet: .. Patient slides in Fig. were fixed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4’,6-diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [ ]. .. EpCAM (Thermo, 14-9326-82) was included in the pan-cytokeratin cocktail mixture to make an “EPI-cocktail”.

    Incubation:

    Article Title: Longitudinal tracking of circulating rare events in the liquid biopsy of stage III–IV non-small cell lung cancer patients
    Article Snippet: Slides were processed at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA). .. Briefly, cells were fixed with paraformaldehyde prior to incubation with 2.5 ug/ml of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA) and 100 ug/ml of a goat anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, PA), permeabilized using 100% cold methanol, followed by an antibody cocktail consisting of mouse IgG1/IgG2a anti-human cytokeratin (CK) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2; C2562, Sigma, St. Louis, MO), mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA), mouse anti-human CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC), and rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA). .. Lastly, slides were incubated with Alexa Fluor® 555 goat anti-mouse IgG1 antibody (A21127, Invitrogen, Carlsbad, CA) and 4′,6-diamidino-2-phenylindole (DAPI; D1306, ThermoFisher) prior to mounted with a glycerol-based aqueous mounting media.

    Article Title: GeTMoR: Simultaneous genomic, transcriptomic, and morphological profiling of rare single cells
    Article Snippet: .. Conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), rabbit IgG anti-human vimentin (Vim or V) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA, USA), and DAPI were also added to the primary incubation buffers. .. Slides were then washed in 2% BSA in PBS and incubated at room temperature for 10 minutes with Alexa Fluor 555 goat anti-mouse IgG1 antibody (A21127, Invitrogen, Carlsbad, CA, USA), conjugated mouse anti-human CD45 Alexa Fluor 647, VIM, and DAPI.

    Article Title: Longitudinal tracking of circulating rare events in the liquid biopsy of stage III-IV non-small cell lung cancer patients.
    Article Snippet: Slides were processed at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA). .. Briefly, cells were fixed with paraformaldehyde prior to incubation with 2.5 ug/ml of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA) and 100 ug/ml of a goat anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, PA), permeabilized using 100% cold methanol, followed by an antibody cocktail consisting of mouse IgG1/IgG2a anti-human cytokeratin (CK) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2; C2562, Sigma, St. Louis, MO), mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA), mouse anti-human CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC), and rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA). .. Lastly, slides were incubated with Alexa Fluor® 555 goat anti-mouse IgG1 antibody (A21127, Invitrogen, Carlsbad, CA) and 4′,6-diamidino-2-phenylindole (DAPI; D1306, ThermoFisher) prior to mounted with a glycerol-based aqueous mounting media.



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    Fig. 1 Large tumor cells are found in bone marrow aspirate of late-stage prostate cancer patients. A Percentage of patients with matched peripheral blood and bone marrow samples with at least 1 CTC-IGC present in liquid biopsy. B Representative images of a typical CTC (left) and CTC-IGCs (middle and right) found in bone marrow aspirate. Far right image depicts two (n = 2) CTC-IGC cells that are EPI-negative but are confirmed to be tumor derived (see Fig S2A). Scale bars set to 15 µM. C PFS from patients with (teal) or without (red) at least one CTC-IGC found in bone marrow samples. D Representative image of typical CTC found in bone marrow with merged and DAPI channels (top) and its genomic copy number profile (bottom). E Representative image of CTC-IGC found in bone marrow with merged and DAPI images (top) and its genomic copy number profile (bottom). F Representative image of mono-nucleated CTC-IGC found in bone marrow with merged (pan- epithelial markers in red, <t>CD45</t> in green, vimentin in white, and DAPI in blue) and DAPI images (top) and its genomic copy number profile (bottom). Scale bars are set to 15 µM in D–F.
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    Image Search Results


    Fig. 1 Large tumor cells are found in bone marrow aspirate of late-stage prostate cancer patients. A Percentage of patients with matched peripheral blood and bone marrow samples with at least 1 CTC-IGC present in liquid biopsy. B Representative images of a typical CTC (left) and CTC-IGCs (middle and right) found in bone marrow aspirate. Far right image depicts two (n = 2) CTC-IGC cells that are EPI-negative but are confirmed to be tumor derived (see Fig S2A). Scale bars set to 15 µM. C PFS from patients with (teal) or without (red) at least one CTC-IGC found in bone marrow samples. D Representative image of typical CTC found in bone marrow with merged and DAPI channels (top) and its genomic copy number profile (bottom). E Representative image of CTC-IGC found in bone marrow with merged and DAPI images (top) and its genomic copy number profile (bottom). F Representative image of mono-nucleated CTC-IGC found in bone marrow with merged (pan- epithelial markers in red, CD45 in green, vimentin in white, and DAPI in blue) and DAPI images (top) and its genomic copy number profile (bottom). Scale bars are set to 15 µM in D–F.

    Journal: Oncogene

    Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance.

    doi: 10.1038/s41388-024-03212-z

    Figure Lengend Snippet: Fig. 1 Large tumor cells are found in bone marrow aspirate of late-stage prostate cancer patients. A Percentage of patients with matched peripheral blood and bone marrow samples with at least 1 CTC-IGC present in liquid biopsy. B Representative images of a typical CTC (left) and CTC-IGCs (middle and right) found in bone marrow aspirate. Far right image depicts two (n = 2) CTC-IGC cells that are EPI-negative but are confirmed to be tumor derived (see Fig S2A). Scale bars set to 15 µM. C PFS from patients with (teal) or without (red) at least one CTC-IGC found in bone marrow samples. D Representative image of typical CTC found in bone marrow with merged and DAPI channels (top) and its genomic copy number profile (bottom). E Representative image of CTC-IGC found in bone marrow with merged and DAPI images (top) and its genomic copy number profile (bottom). F Representative image of mono-nucleated CTC-IGC found in bone marrow with merged (pan- epithelial markers in red, CD45 in green, vimentin in white, and DAPI in blue) and DAPI images (top) and its genomic copy number profile (bottom). Scale bars are set to 15 µM in D–F.

    Article Snippet: Patient slides in Fig. 1 were fixed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4’,6- diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [46].

    Techniques: Derivative Assay